8 tools
Calculates the optimal annealing temperature for PCR thermal cycles based on primer sequences, ensuring efficient DNA amplification by determining the temperature at which the primers will base-pair with the template DNA effectively. Helps users in molecular biology, genetics, and biochemistry to design and optimize their PCR experiments. Researchers, students, and professionals working with genetic material can use this tool to improve the specificity and sensitivity of their DNA amplification reactions by selecting appropriate annealing temperatures for their primers.
Calculates cell culture growth rate and doubling time based on initial cell count and final cell count, as well as the number of hours or days the culture has been growing. Researchers, scientists, and students in biology, microbiology, and related fields use it to quickly determine how fast a cell population is increasing, which is crucial for understanding cellular processes, optimizing experiments, and monitoring growth dynamics.
Calculates protein concentration or dilution from spectroscopic absorbance measurement using a simple formula based on Beer's Law. Users input absorbance values, molar extinction coefficient, and path length to find protein concentration in grams per liter (g/L) or micrograms per milliliter (μg/mL). Researchers, students, and professionals in molecular biology, biochemistry, and related fields use this tool to accurately measure and quantify proteins in solutions. It's invaluable for experiments requiring precise protein concentrations, such as Western blots, ELISAs, and other biochemical assays.
Calculates bacterial growth by simulating population increase over time, based on initial population size and reproduction rate. Users input key parameters, then view visual representation of growth pattern. Suitable for biology students, microbiologists, or anyone interested in understanding bacterial reproduction dynamics. Helps visualize concepts such as exponential growth and doubling times in a practical, interactive manner.
Calculates the concentration of DNA, RNA, or custom oligonucleotide sequences using inputs such as mass, absorbance, or volume measurements. This tool simplifies the process for researchers and students by providing quick and accurate concentration values based on standard biological techniques. Ideal for anyone needing to measure DNA concentration for experiments or studies in molecular biology, genetics, and related fields.
Calculates the optimal mass of insert and vector for cloning reactions based on desired yield and efficiency factors. User inputs target plasmid size, insert length, and reaction conditions to receive precise ligation parameters. Biologists, researchers, and students involved in molecular biology and genetic engineering would use this tool to ensure successful DNA ligation experiments. It helps in optimizing experimental design by providing accurate mass ratios for the components needed, reducing trial-and-error and saving time and resources in laboratory settings.
Calculates log reduction and percentage reduction of microorganisms following treatment application. This tool provides a structured method for determining the efficacy of disinfectants or sterilization agents against microbial populations. Users input data regarding initial and final concentrations, allowing the calculator to derive the quantitative measure of kill rate in standard logarithmic units. The resulting calculations help quantify how effectively a specific antimicrobial agent reduces the viable count of various pathogens. Scientists and laboratory professionals utilize this resource for disinfectant validation protocols. It is essential for quality assurance testing where documenting microbial inactivation levels is critical.
Calculates the necessary volumes for preparing cell suspensions of specific concentrations. This tool helps users determine how much initial solution must be diluted to reach a desired final concentration of cells. By inputting the starting volume, the target concentration, and the required dilution factor, it provides precise measurements needed for accurate lab preparation. The calculator follows standard mathematical principles used in biological sample processing to ensure proper ratios are maintained across different experimental setups. Researchers, students, and laboratory technicians use this utility when preparing cultures or samples for various assays. Whether performing flow cytometry, running viability tests, or seeding cells onto plates, achieving the exact concentration is critical for reliable results.
